REVEALING THE PRESENCE OF THE EAST ASIAN LEAFHOPPER TAUTONEURA POLYMITUSA (HEMIPTERA: AUCHENORRHYNCHA: CICADELLIDAE: TYPHLOCYBINAE) IN SERBIA THROUGH DNA BARCODING

Authors

  • Andrea Kosovac Department of Plant Pests, Institute for Plant Protection and Environment, 11000 Belgrade, Serbia
  • Marko Šćiban Bird Protection and Study Society of Serbia, Partizanskih baza 6/43, 21000 Novi Sad, Serbia
  • Ivan Pančić Zrenjanin, Serbia
  • Mária Tóth MTA-ELTE-MTM, Hungarian Academy of Sciences - Eötvös University. Hungarian Natural History Museum, H-1117, Budapest, Hungary
  • László Ronkay Hungarian Natural History Museum, H-1088 Budapest, Hungary
  • András Orosz Hungarian Natural History Museum, H-1088 Budapest, Hungary

DOI:

https://doi.org/10.5281/zenodo.3878684

Keywords:

Tautoneura polymitusa, DNA barcoding, Serbia, first record

Abstract

After the first observations of cicadas of the subfamily Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae), a specific white-orange-red coloration in Zrenjanin and Melenci in 2017 and 2018, four females of the same habitus were collected in August 2019 in Belgrade and Special Nature Reserve “Deliblato Sands”. External morphology analyzes indicated that they are most similar to the East Asian invasive species present in Europe, Tautoneura polymitusa Oh & Jung 2016. Due to the lack of sampled males, reliable species determination required the use of molecular methods. Since no nucleotide sequences of the barcoding region of the COI gene of the T. polymitus species are available in gene banks, specimens of males collected in Budapest at the site of the first find of the species in Europe were attached to the genetic identification. After successful multiplication and sequencing of the COI barcoding region, 100% genetic matching of the sequences of the analyzed females from Serbia and males from Hungary was determined. The obtained results confirm the presence of allochthonous cicada T. rolymitus in Serbia and indicate the importance of the barcoding method which in the context of biodiversity research successfully overcomes sampling problems such as small number of specimens or absence of males.

References

Chetverikov, P. E., Cvrković, T., Makunin, A., Sukhareva, S., Vidović, B., & Petanović, R. (2015). Experimental and Applied Acarology, 67(2): 219-245; D’Urso, V., Sánchez, I., & Bella, S. (2019). Phytoparasitica, 47(1), 1-16; Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). Molecular marine biology and biotechnology, 3, 294-299; Janković, Lj (1975). Zbornik radova o fauni Srbije, I: 85-209; Janković, Lj. (1978). Zbornik radova o fauni Srbije, II: 133-153; Janković, Lj., & Papović, R. (1985). Zbornik radova o fauni Srbije, III: 111-147; Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I., & Jović, J. (2018). PloS one, 13(5); Seljak, G. (2018). Acta Entomologica Slovenica, 26(2), 181-194; Sheth, B. P., & Thaker, V. S. (2017). Genome, 60(7), 618-628; Stepanović, S., Kosovac, A., Krstić, O., Jović, J. & Toševski, I. (2016). Insect science, 23(4), 638-648; Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei M., & Kumar S. (2011). Molecular Biology and Evolution, 28, 2731-2739; Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). Nucleic Acids Research, 22, 4673-4680; Tóth, M., Orosz, A., & Rédei, D. (2017). Zootaxa, 4311(1), 137-144.

Downloads

Published

2020-06-05

Issue

Vol. 25 No. 1 (2020)

Section

Short communications

License

Copyright: © 2019 The ENTOMOLOGICAL SOCIETY OF SERBIA Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

How to Cite

REVEALING THE PRESENCE OF THE EAST ASIAN LEAFHOPPER TAUTONEURA POLYMITUSA (HEMIPTERA: AUCHENORRHYNCHA: CICADELLIDAE: TYPHLOCYBINAE) IN SERBIA THROUGH DNA BARCODING. (2020). Acta Entomologica Serbica, 25(1), 83-86. https://doi.org/10.5281/zenodo.3878684